Effect of Co-EDTA on hematological parameters in immature mice

As inorganic and complex compounds cobalt is used as nutritional supplement, preservative, in drinks, cosmetics, as therapeutic agent for treating different diseases. The aim of the present study is to investigate the effect of Co-EDTA on the hematological parameters of immature mice. Pregnant mice in late gestation were subjected to Co-EDTA treatment at daily doses of 75 mg/kg and the exposure continued until days 18 and 25 of the newborn pups. The compound was obtained from drinking water. Hematological parameters and serum iron (Fe) content were measured. Preliminary results show reduced red blood cell count (RBC), hemoglobin content (Hb), hematocrit (Hct) and mean cell volume (MCV) and increased MCH and MCHC in treated mice. Serum Fe was elevated in d18 mice and significantly reduced in d25 animals compared to agematched controls. Fe content was in good correlation with RBC, Hb, Hct, MCV and showed strong inverse relation with MCH and MCHC in d18 mice which corresponds to the increased values of the latter parameters in the treated group. Results indicate that treatment of immature mice with Co-EDTA induces significant hematological changes which probably alter their hemorheological properties as well.